Development and Validation of a Liquid Chromatography–Tandem Mass Spectrometry Method for the Identification and Quantification of JWH-018, JWH-073, JWH-019, and JWH-250 in Human Whole Blood
NMS Labs has published a white paper on development and validation of an LC/MS-MS method for synthetic cannabinoids JWH-018, JWH-073, JWH-019 and JWH-250. The paper will appear in Journal of Analytical Toxicology, Vol. 35, September 2011. Abstract is included below.
The paper was written by a highly skilled team of toxicologists and scientists from NMS Labs. This team is comprised of:
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ABSTRACT: A sensitive and specific method for the quantification of JWH-018, JWH-073, and JWH-250 and the qualitative identification of JWH-019 in whole blood was developed and validated. Samples fortified with JWH-018-d9 and JWH-073-d9 underwent liquid–liquid extraction and were analyzed by liquid chromatography–positive ion electrospray ionizationtandem mass spectrometry. Two transitions were monitored for all analytes except JWH-250, for which there was only one available transition. JWH-019 did not meet the stringent requirements for quantitative analysis, and thus this method is only appropriate for the qualitative identification of this compound in whole blood. The linear range was 0.1–20 μg/L for all quantitative analytes. The maximum average withinand between-run imprecision was 7.9% and 10.2%, respectively, and all controls quantified within 8.2% of target concentrations. Process efficiency, a measurement that takes into effect extraction efficiency and matrix effect, was ≥ 32.0% for all quantitative analytes; similar results were obtained for the deuterated internal standards. All analytes were stable at room, refrigerated, and frozen temperatures for at least 30 days. The method was used to quantify JWH-018 and JWH-073 in a blood specimen collected from a person known to have used an herbal incense blend containing these substances.
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