Bingfang Yue, Tiedong Guo, Loan Nguyen
NMS Labs, Willow Grove, PA

Catecholamines (dopamine, norepinephrine, and epinephrine) act as neurotransmitters or hormones at both central and peripheral levels, in both neuronal and non-neuronal tissues. The involvement of catecholamines in multiple regulatory systems and metabolic processes supports their important role as biomarkers for the clinical diagnosis, therapy, and prognosis of several neuroendocrine and cardiovascular disorders. 

Catecholamines exist in biological samples at extremely low concentrations and are chemically unstable, prone to spontaneous oxidation and decompose easily at high pH. The quantification of catecholamines demands specific and very sensitive bioanalytical methods. Current standard method of choice is HPLC with electrochemical (EC) detection, despite time consuming sample preparation, long chromatographic runtime, and low sensitivity severely limiting its clinical use. 

NMS Labs developed a simple, fast and sensitive 2D-LC-MS/MS method to measure three catecholamines in routine clinical laboratory setting.

NMS Labs presented at The Association for Mass Spectrometry: Applications to the Clinical Laboratory, Inc. (MSACL) in March 2014. The presentation is summarized in a research poster. Request a PDF of the poster.