NMS Labs has published a white paper on development and validation of an LC/MS-MS method for synthetic cannabinoids JWH-018, JWH-073, JWH-019 and JWH-250. The paper will appear in Journal of Analytical Toxicology, Vol. 35, September 2011. Abstract is included below.

The paper was written by a highly skilled team of toxicologists and scientists from NMS Labs. This team is comprised of:

  • Sherri L. Kacinko
  • Allan Xu
  • Joseph W. Homan
  • Matthew M. McMullin
  • Donna M. Warrington
  • Barry K. Logan

Please e-mail us if you would like a PDF copy of the published article.

ABSTRACT: A sensitive and specific method for the quantification of JWH-018, JWH-073, and JWH-250 and the qualitative identification of JWH-019 in whole blood was developed and validated. Samples fortified with JWH-018-d9 and JWH-073-d9 underwent liquid–liquid extraction and were analyzed by liquid chromatography–positive ion electrospray ionizationtandem mass spectrometry. Two transitions were monitored for all analytes except JWH-250, for which there was only one available transition. JWH-019 did not meet the stringent requirements for quantitative analysis, and thus this method is only appropriate for the qualitative identification of this compound in whole blood. The linear range was 0.1–20 μg/L for all quantitative analytes. The maximum average withinand between-run imprecision was 7.9% and 10.2%, respectively, and all controls quantified within 8.2% of target concentrations. Process efficiency, a measurement that takes into effect extraction efficiency and matrix effect, was ≥ 32.0% for all quantitative analytes; similar results were obtained for the deuterated internal standards. All analytes were stable at room, refrigerated, and frozen temperatures for at least 30 days. The method was used to quantify JWH-018 and JWH-073 in a blood specimen collected from a person known to have used an herbal incense blend containing these substances.

NMS Labs is the only independent forensic facility in the nation to be certified by both the ABFT (American Board of Forensic Toxicology) and ANAB-ASCLD/LAB-International (ANSI/ASQ National Accreditation Board-American Society of Crime Lab Directors/Lab Accreditation Board ISO 17025). ISO 17025 accreditation covers the disciplines of Toxicology, DNA, serology and controlled substances. We diligently maintain and exceed the high standards set by these organizations, so that you can have the utmost confidence in your testing results.