Simplified Analysis of 11-Hydroxy-Delta-9-Tetrahydrocannabinol and 11-Carboxy-Delta-9-Tetrahydrocannabinol in Human Meconium: Method Development and Validation

Marykathryn Tynon1,2,3, Marcellino Porto1 and Barry K. Logan1,2,3

1NMS Labs, 3701 Welsh Rd, Willow Grove, PA 19090, USA, 2Forensic Science Program, Arcadia University, Glenside, PA, USA, and 3The Center for Forensic Science Research and Education, The Fredric Rieders Family Renaissance Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA

 

The article Simplified Analysis of 11-Hydroxy-Delta-9-Tetrahydrocannabinol and 11-Carboxy-Delta-9-Tetrahydrocannabinol in Human Meconium: Method Development and Validationappears in the Journal of Analytical Toxicology 2014;1–6 doi:10.1093/jat/bku107. Abstract is included below.

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ABSTRACT: We describe the development of a sensitive analytical method for the analysis of 11-hydroxy-delta-9-tetrahydrocannabinol (11-OH-THC) and 11-carboxy-delta-9-tetrahydrocannabinol (THCC) in meconium using a gas chromatography–mass spectrometry (GC/MS) platform. The method was validated according to protocols, which included assessment of accuracy, precision, robustness, stability in meconium and in-process stability, interference and sensitivity and specificity. The method consists of a solid phase extraction with alkaline hydrolysis and derivatization of the analytes with N, O-Bis(trimethylsilyl)trifluoroacteamide, followed by GC/MS analysis using selected ion monitoring. The method uses deuterated internal standards for both analytes. Calibration curves had r2 values >0.998, and extraction efficiency was determined to be 84.7% for THCC and 78.6% for 11-OH-THC. The detection limit for both analytes was 5 ng/g. This confirmatory method was successfully applied to 183 meconium samples that had screened positive by enzyme-linked immunosorbent assay, and 67.2% were confirmed for THCC, and 2.2% were confirmed positive for 11-OH-THC. The mean (SD) and median (range) THCC (n 5 123) concentrations detected were 55.0 ng/g (+59.0) and 33.75 ng/g (5–265 ng/g), while the mean and median (range) for 11-OH-THC (n 5 4) concentrations were 8.25 ng/g (+4.71) and 6.5 ng/g (5–15 ng/g).

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